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Paneth cells constitute the niche for Lgr5 stem cells in intestinal crypts

LGR5型 干细胞 潘尼斯电池 细胞生物学 生物 成体干细胞 肠上皮 肠内分泌细胞 细胞分化 多能干细胞 上皮 癌症干细胞 祖细胞 小肠 生物化学 遗传学 基因 内分泌系统 激素
作者
Toshiro Sato,Johan H. van Es,Hugo J.G. Snippert,Daniel E. Stange,Robert G. Vries,Maaike van den Born,Nick Barker,Noah F. Shroyer,Marc van de Wetering,Hans Clevers
出处
期刊:Nature [Nature Portfolio]
卷期号:469 (7330): 415-418 被引量:2327
标识
DOI:10.1038/nature09637
摘要

Paneth cells, specialized cells found in the intestinal epithelium, are known to protect stem cells by producing bactericidal compounds. Now another crucial function is reported: they provide the essential niche signals (EGF/TGFα, Notch and Wnt) for Lgr5-expressing stem cells in the small intestine. Multipotent stem cells expressing Lgr5 generate all intestinal epithelium cell types — Paneth cells included. Stem-cell niches are often seen as pre-existing sites to which stem cells migrate; this work shows that intestinal stem cells receive niche support from their own progeny. Multipotent stem cells expressing Lgr5 are known to generate all cell types of the intestinal epithelium (enterocytes, goblet cells, Paneth cells and enteroendocrine cells). A new study shows that Paneth cells have an essential role for intestinal crypt and stem cell maintenance by supplying essential niche signals to the Lgr5-expressing cells. Homeostasis of self-renewing small intestinal crypts results from neutral competition between Lgr5 stem cells, which are small cycling cells located at crypt bottoms1,2. Lgr5 stem cells are interspersed between terminally differentiated Paneth cells that are known to produce bactericidal products such as lysozyme and cryptdins/defensins3. Single Lgr5-expressing stem cells can be cultured to form long-lived, self-organizing crypt–villus organoids in the absence of non-epithelial niche cells4. Here we find a close physical association of Lgr5 stem cells with Paneth cells in mice, both in vivo and in vitro. CD24+ Paneth cells express EGF, TGF-α, Wnt3 and the Notch ligand Dll4, all essential signals for stem-cell maintenance in culture. Co-culturing of sorted stem cells with Paneth cells markedly improves organoid formation. This Paneth cell requirement can be substituted by a pulse of exogenous Wnt. Genetic removal of Paneth cells in vivo results in the concomitant loss of Lgr5 stem cells. In colon crypts, CD24+ cells residing between Lgr5 stem cells may represent the Paneth cell equivalents. We conclude that Lgr5 stem cells compete for essential niche signals provided by a specialized daughter cell, the Paneth cell.
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