生物
脱落酸
MYB公司
拟南芥
转基因
基因
基因表达
转录因子
突变体
发起人
基因表达调控
报告基因
细胞生物学
分子生物学
遗传学
作者
Hiroshi Abe,Takeshi Urao,Takuya Ito,Motoaki Seki,Kazuo Shinozaki,Kazuko Yamaguchi‐Shinozaki
出处
期刊:The Plant Cell
[Oxford University Press]
日期:2002-12-19
卷期号:15 (1): 63-78
被引量:1902
摘要
In Arabidopsis, the induction of a dehydration-responsive gene, rd22, is mediated by abscisic acid (ABA). We reported previously that MYC and MYB recognition sites in the rd22 promoter region function as cis-acting elements in the drought- and ABA-induced gene expression of rd22. bHLH- and MYB-related transcription factors, rd22BP1 (renamed AtMYC2) and AtMYB2, interact specifically with the MYC and MYB recognition sites, respectively, in vitro and activate the transcription of the beta-glucuronidase reporter gene driven by the MYC and MYB recognition sites in Arabidopsis leaf protoplasts. Here, we show that transgenic plants overexpressing AtMYC2 and/or AtMYB2 cDNAs have higher sensitivity to ABA. The ABA-induced gene expression of rd22 and AtADH1 was enhanced in these transgenic plants. Microarray analysis of the transgenic plants overexpressing both AtMYC2 and AtMYB2 cDNAs revealed that several ABA-inducible genes also are upregulated in the transgenic plants. By contrast, a Ds insertion mutant of the AtMYC2 gene was less sensitive to ABA and showed significantly decreased ABA-induced gene expression of rd22 and AtADH1. These results indicate that both AtMYC2 and AtMYB2 proteins function as transcriptional activators in ABA-inducible gene expression under drought stress in plants.
科研通智能强力驱动
Strongly Powered by AbleSci AI