核糖核酸酶P
核糖核酸
掷骰子
生物
核糖核酸酶H
核糖核酸酶Ⅲ
分子生物学
核糖核酸酶
DNA
信使核糖核酸
生物化学
RNA干扰
基因
作者
Nicole M. Nichols,Dongxian Yue
标识
DOI:10.1002/0471142727.mb0313s84
摘要
Abstract Ribonucleases (RNases) with different sequence or structural specificities are used for a variety of analytical purposes, including RNA sequencing, mapping, and quantitation. The development of RNase protection assays, structural determination assays, and the production of small interfering RNAs (siRNA) employed in RNA interference (RNAi) experiments has depended on the unique substrate specificities of commercially available RNases, including RNases A, I, T1, V1, HI, III, and Dicer. One very common application for high purity RNase A is also presented in this unit and involves hydrolyzing RNA that contaminates DNA preparations. RNase HII and the placental RNase inhibitor are also discussed. Curr. Protoc. Mol. Biol . 84:3.13.1‐3.13.8. © 2008 by John Wiley & Sons, Inc.
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