Revised Preparation of a Mimetic Tissue Model for Quantitative Imaging Mass Spectrometry

A revised methodology is presented for the preparation of a mimetic tissue model for use in quantitative imaging mass spectrometry \(IMS).Tissue homogenates spiked with known concentrations of a compound standard are serially added to a mold and are snap frozen.The resulting mimetic tissue plug is then cryosectioned and thaw-mounted on the same slide as the tissue\(s) to be imaged and quanti ed.From here, the sample tissue\(s) and mimetic tissue model undergo identical sample preparation conditions for IMS.Once IMS is performed, the known analyte concentrations can be correlated with the average intensity of each layer of the mimetic model to generate a calibration curve.This standard curve can then be used to estimate the quantity of analyte that is present in the tissue\(s) of interest.The matrix-matched standards applied in this approach account for common IMS issues such as ion suppression and extraction e ciency.