Simple and Highly Selective Electrochemical Sensor Constructed Using Silver Molybdate Nano-Wire Modified Electrodes for the Determination of Oxidative Stress Biomarker in Blood Serum and Lens Cleaning Solution

In this work, a simple synthesis of (silver molybdate) Ag2MoO4 nanowires was developed by varying the pH. By adjusting the pH, agglomeration of each Ag2MoO4 nanowires were seen and electrochemical performance over each pH was investigated. To construct Ag2MoO4 nanowires, this process involves a co-precipitation assisted ultrasound reaction. The as-synthesized Ag2MoO4 was physically characterized using SEM, elemental mapping, EDX, and XRD analysis. Ag2MoO4 modified GCE was developed to determine a hydrothermal free and urea-free assisted synthesis. H2O2 plays a major role in the physiological process and it's an oxidative stress biomarker. Here we develop an electrochemical detection of enzyme-free H2O2 by amperometric method. The results obtained by modifying the electrode Ag2MoO4@pH-1 (AM@1) showed a good electrochemical reduction over current when compared to the bare electrode and Ag2MoO4@pH 2 to 5. LOD was calculated to be 5.42 nM with a linear range from 0.015 to 799.2 μM. The selectivity of sensor was analysed based on AM@1 modified electrode and also, the modified AM@1/GCE sensor has been practically applied in biological samples. Further, AM@1 showed a greater selectivity towards H2O2 in presence of some other biochemicals. Moreover, AM@1 exhibits advantages such as good stability, reproducibility, and repeatability.