Production of Crude Xylanase From Trichoderma sp. Using Reutealis trisperma Exocarp Substrate in Solid State Fermentation

Abstract Reutealis trisperma exocarp is a waste containing hemicellulose, cellulose, and lignin which can be used to produce xylanase enzymes. Reutealis trisperma exocarp contains 44.48% of hemicellulose, a polymer of xylan. The xylanase is an enzyme that can be utilized for hydrolyzing hemicellulose (xylan) to xylose. Xylanase can be produced by solid-state fermentation using isolate Trichoderma sp and Reutealis trisperma exocarp as substrate. This research aims to determine the fermentation time and substrate concentration that produce the highest xylanase activity using Trichoderma sp. In this study, the crude extract enzyme was evaluated on xylanase activity, protein content and specific activity at various fermentation times of 12, 24, 36, 48, 60 h using substrate concentrations of 2%, 4%, 6%, and 8%. The highest xylanase activity was observed with the substrate concentration of 8% at the 60 hour fermentation time. In this process, 672.039 U/mL of xylanase activity, protein content around 0.590 mg / mL and 1137.638 U/mg of the specific activity were produced.