前药
化学
内吞作用
过氧化物酶
生物相容性
癌症治疗
癌细胞
细胞凋亡
生物化学
酶
癌症研究
纳米技术
细胞
癌症
材料科学
生物
有机化学
遗传学
作者
Qian Liang,Juqun Xi,Xuejiao J. Gao,Ruofei Zhang,Yili Yang,Xingfa Gao,Xiyun Yan,Lizeng Gao,Kelong Fan
出处
期刊:Nano Today
[Elsevier]
日期:2020-12-01
卷期号:35: 100935-100935
被引量:124
标识
DOI:10.1016/j.nantod.2020.100935
摘要
Enzyme-mediated activation of prodrug in tumor cells is an attractive strategy for targeted cancer therapy. Of note, natural peroxidase and indole-3-acetic acid (IAA) have been shown as a promising enzyme-prodrug combination. However, insufficient endogenous peroxidase activity and the difficulty in selectively expressing exogenous peroxidase in tumor cells have severely limited its antitumor efficiency. The discovery of nanomaterials with peroxidase-like activity (nanozyme), particularly the metal-free nanozymes that possesses high stability and good biocompatibility, bring a new opportunity to overcome these challenges. Here, under the guidance of theoretical calculations, we developed a metal-free phosphorous and nitrogen dual-doped porous hollow carbon sphere nanozyme (PNCNzyme) with robust peroxidase-like activity in acidic environment. Based on this metal-free nanozyme, we developed a nanozyme-IAA activation strategy that activated IAA to produce abundant ROS and trigger tumor cell apoptosis. We also introduced folate (FA) onto the nanozyme to enhance its tumor targeting and endocytosis efficacy by tumor cells. Upon administrated to mice, the [email protected] effectively accumulated in the xenografts derived from cervical cancer cells, catalyzed the production of ROS, and induced apoptosis. Taken together, these data demonstrate that the nanozyme-IAA combination is an effective enzyme-prodrug activation strategy for tumor targeted catalytic therapy.
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