生化工程
计算生物学
蛋白质表达
生物技术
表达式(计算机科学)
生物
定向进化
合成生物学
计算机科学
工程类
生物化学
基因
突变体
程序设计语言
作者
Lukas Rieder,Nico Teuschler,Katharina Ebner,Anton Glieder
标识
DOI:10.1002/9783527813780.ch1_3
摘要
Since the introduction of the first biotechnological processes, the demand for product quantity and quality was steadily increasing. While many protein production processes for valuable speciality enzymes and valuable biocatalysts are carried out with bacterial expression hosts, this chapter focuses on yeasts and fungi as alternatives for the production of low cost enzymes and enzymes where successful expression relies on eukaryotic host cell machineries. One focus is on molecular biological tools such as promoter engineering and strain evolution. However, in spite of many useful individual methods that were applied to individual products, in general, successful implementations of modern expression strategies rely on optimized combinations of complementary technologies as outlined in this chapter.
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