免疫分型
免疫球蛋白轻链
流式细胞术
骨髓
病理
细胞仪
淋巴瘤
B细胞
CD38
CD19
医学
分子生物学
生物
免疫学
川地34
抗体
遗传学
干细胞
作者
Siba El Hussein,Wei Wang,Sa A. Wang,Hong Fang,Sofía Garcés,Mehrnoosh Tashakori,Kirill A. Lyapichev,Lianqun Qiu,Xiaoqiong Wang,Sanam Loghavi,L. Jeffrey Medeiros,Pramoda Challagundla,Jeffrey L. Jorgensen
标识
DOI:10.1016/j.leukres.2021.106704
摘要
Light-chain restricted hematogones (LCR HGs) detected by flow cytometry analysis can mimic bone marrow involvement by B-cell lymphoma. This phenomenon can present a diagnostic pitfall and negatively impact patient management, as misinterpretation may upgrade disease stage. In this study, we characterized the immunophenotype of LCR HGs with an aim to differentiate them from B-cell lymphoma. We analyzed 24 patients with LCR HGs, 12 (50 %) were kappa light chain restricted and 12 (50 %) were lambda light chain restricted. LCR HGs account for 51 % (range, 1.5%-99%) of B cells, and 0.5 % (range, 0.1%-3.7%) of total cells. In 15 patients in whom multiple specimens were analyzed, 10 (67 %) showed persistent LCR HGs in more than 1 specimen, and the duration of the light chain restriction ranged from 4 months to 2 years. Among 24 patients, 4 (16.6 %) cases were concurrently involved by B-cell lymphoma/myeloma in addition to LCR HGs. With the exception of light chain restriction, LCR HGs showed a similar immunophenotype as normal HGs and had a distinct location on the CD45/Side Scatter (SSC) plot. They were also consistently positive for CD10, CD19, CD38 (bright), CD43, and CD200. CD20 expression showed a spectrum from dim/negative to positive.
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