纳米传感器
费斯特共振能量转移
赭曲霉毒素A
荧光
能量转移
化学
分析化学(期刊)
纳米技术
材料科学
色谱法
物理
光学
食品科学
分子物理学
真菌毒素
作者
Benchao Su,Zhong Zhang,Zhichang Sun,Zongwen Tang,Xiaoxia Xie,Qi Chen,Hongmei Cao,Xi Yu,Youlong Xu,Xing Liu,Bruce D. Hammock
标识
DOI:10.1016/j.jhazmat.2021.126838
摘要
Ochratoxin A (OTA) contamination in food is a serious threat to public health. There is an urgent need for development of rapid and sensitive methods for OTA detection, to minimize consumer exposure to OTA. In this study, we constructed two OTA-specific fluonanobodies (FluoNbs), with a nanobody fused at the carboxyl-terminal (SGFP-Nb) or the amino-terminal (Nb-SGFP) of superfolder green fluorescence protein. SGFP-Nb, which displayed better fluorescence performance, was selected as the tracer for OTA, to develop a FluoNb-based nanosensor (FN-Nanosens) via the fluorescence resonance energy transfer, where the SGFP-Nb served as the donor and the chemical conjugates of OTA-quantum dots served as the acceptor. After optimization, FN-Nanosens showed a limit of detection of 5 pg/mL, with a linear detection range of 5-5000 pg/mL. FN-Nanosens was found to be highly selective for OTA and showed good accuracy and repeatability in recovery experiments using cereals with various complex matrix environments. Moreover, the contents of OTA in real samples measured using FN-Nanosens correlated well with those from the liquid chromatography with tandem mass spectrometry. Therefore, this work illustrated that the FluoNb is an ideal immunosensing tool and that FN-Nanosens is reliable for rapid detection of OTA in cereals with ultrahigh sensitivity.
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