小RNA
甲状腺癌
医学
外体
转移
内科学
微阵列
肿瘤科
生物标志物
实时聚合酶链反应
淋巴结
癌症研究
病理
生物信息学
甲状腺
癌症
生物
基因表达
微泡
基因
遗传学
作者
Wenjie Chen,Genpeng Li,Zhihui Li,Junfei Zhu,Tao Wei,Jianyong Lei
出处
期刊:Endocrine
[Springer Nature]
日期:2021-12-01
卷期号:75 (3): 846-855
被引量:9
标识
DOI:10.1007/s12020-021-02949-x
摘要
PurposeThe early diagnosis of lymph node metastasis (LNM) in papillary thyroid carcinoma (PTC) is clinically important, as it can aid in treatment decision-making and improve prognosis. In the present study, we aimed to identify whether plasma exosomal miRNAs could be potential diagnostic markers of LNM in PTC.MethodsProfiles of plasma exosomal miRNAs were screened using miRNA microarrays. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was performed in the validation and diagnostic sets to select candidate exosomal miRNAs. Finally, receiver operating characteristic (ROC) curves were generated to evaluate the efficiency of target exosomal miRNAs in distinguishing PTC-N1 patients from PTC-N0 patients.ResultsIn total, 197 miRNAs were found to be differentially expressed in the testing set. Based on the qRT-PCR results, the expression of miR-6774-3p (p < 0.001) and miR-6879-5p (p < 0.001) in the PTC-N1 patients was significantly higher than that in the controls. The AUC values of plasma exosomal miR-6774-3p (0.802; 95% CI, 0.724–0.879) and miR-6879-5p (0.787; 95% CI, 0.706–0.867) and their combination (0.914; 95% CI, 0.865–0.962) were higher than those of the total miRNAs directly isolated from plasma. Moreover, the expression of exosomal miRNAs was stable after treatment with RNase A, prolonged incubation, or repeated freezing and thawing.ConclusionsThe two plasma exosomal miRNAs (miR-6774-3p and miR-6879-5p) and their combination could serve as new promising biomarkers for the diagnosis of LNM in PTC patients.
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