Increasingly, nanotechnology has the potential to be applied in disease detection or treatment. Nanotechnology advancements have resulted in novel and improved biomedical nanomaterials. Liposomes, polymeric micelles, graphene, carbon nanotubes, quantum dots, ferro ferric oxide nanoparticles, gold nanoparticles (Au NPs), and so on are some of the most commonly used nanomaterials in biomedical applications. Because of its unique optical, electrical, sensing and biological capabilities, Au NPs have been regarded as the most fascinating nanomaterial. Medical imaging, drug administration and tumour therapy are all possible applications of Au NPs in the early identification and diagnosis and treatment of illnesses. This review focuses on some recent breakthroughs in the management of Au NPs as drug carriers for the intracellular delivery of therapies and as molecular nanoprobes for the detection and monitoring of target molecules.