Land Use Overrides Stream Order and Season in Driving Dissolved Organic Matter Dynamics Throughout the Year in a River Network

Anthropogenic land use has increased nutrient concentrations and altered dissolved organic matter (DOM) character and its bioavailability. Despite widespread recognition that DOM character and its reactivity can vary temporally, the relative influence of land use and stream order on DOM characteristics is poorly understood across seasons and the entire flow regime. We examined DOM character and 28-day bioavailable dissolved organic carbon (BDOC) across a river network to determine the relative roles of land use and stream order in driving variability in DOM character and bioavailability throughout the year. DOM in 1st-order streams was distinct from higher stream orders with lower DOC concentrations, less aromatic (specific ultraviolet absorbance at 254 nm (SUVA254)), more autochthonous (fluorescence index), and more recently produced (β/α) DOM. Across all months, variability in DOM character was primarily explained by land use, rather than stream order or season. Land use and stream order explained the most DOM variation in transitional and winter months and the least during dry months. BDOC was greater in watersheds with less aromatic (SUVA254) and more recent allochthonous DOM (β/α) and more development and impervious surface. With continued development, the bioavailability of DOM in the smallest and most impacted watersheds is expected to increase.