生长素
流出
生物
拟南芥
突变体
细胞生物学
ATP结合盒运输机
膜转运蛋白
运输机
生物化学
基因
作者
Nathan Mellor,Ute Voß,Alexander Ware,George Janes,Duncan Barrack,Anthony Bishopp,Malcolm J. Bennett,Markus Geisler,Darren M. Wells,Leah R. Band
出处
期刊:The Plant Cell
[Oxford University Press]
日期:2022-03-18
卷期号:34 (6): 2309-2327
被引量:18
标识
DOI:10.1093/plcell/koac086
摘要
Members of the B family of membrane-bound ATP-binding cassette (ABC) transporters represent key components of the auxin efflux machinery in plants. Over the last two decades, experimental studies have shown that modifying ATP-binding cassette sub-family B (ABCB) expression affects auxin distribution and plant phenotypes. However, precisely how ABCB proteins transport auxin in conjunction with the more widely studied family of PIN-formed (PIN) auxin efflux transporters is unclear, and studies using heterologous systems have produced conflicting results. Here, we integrate ABCB localization data into a multicellular model of auxin transport in the Arabidopsis thaliana root tip to predict how ABCB-mediated auxin transport impacts organ-scale auxin distribution. We use our model to test five potential ABCB-PIN regulatory interactions, simulating the auxin dynamics for each interaction and quantitatively comparing the predictions with experimental images of the DII-VENUS auxin reporter in wild-type and abcb single and double loss-of-function mutants. Only specific ABCB-PIN regulatory interactions result in predictions that recreate the experimentally observed DII-VENUS distributions and long-distance auxin transport. Our results suggest that ABCBs enable auxin efflux independently of PINs; however, PIN-mediated auxin efflux is predominantly through a co-dependent efflux where co-localized with ABCBs.
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