材料科学
生物传感器
光子上转换
检出限
激发
光子晶体
光电子学
发光
单色
光子学
干扰(通信)
猝灭(荧光)
纳米技术
光学
荧光
化学
电气工程
物理
频道(广播)
工程类
色谱法
作者
Ling Zhang,Songtao Hu,Yang Lu,Bin Jiang,Xiao Liu,Xiaodan Li,Yan Wang,Yuehe Lin,Chenguang Wang,Xiaoteng Jia,Fengmin Liu,Biao Dong,Geyu Lu
标识
DOI:10.1021/acsami.1c21834
摘要
Cholesterol is a vital compound in maintenance for human health, and its concentration levels are tightly associated with various diseases. Therefore, accurate monitoring of cholesterol is of great significance in clinical diagnosis. Herein, we fabricated a noncontact biosensor based on photonic crystal-enhanced upconversion nanoparticles (UCNPs) for highly sensitive and interference-free cholesterol detection. By compounding LiErF4:0.5%Tm3+@LiYF4 UCNPs with poly(methyl methacrylate) (PMMA) photonic crystals (OPCs), we were able to selectively tune the coupling of the photonic band gap to the excitation field and modulate the upconversion (UC) luminescence intensity, given the unique multi-wavelength excitation property of LiErF4:0.5%Tm3+@LiYF4. A 48.5-fold enhancement of the monochromatic red UC emission was ultimately achieved at 980 nm excitation, ensuring improved detection sensitivity. Based on the principle of quenching of the intense monochromic red UC emission by the oxidation products of 3,3',5,5'-tetramethylbenzidine (TMB) yielded from the cholesterol cascade reactions, the biosensor has a detection limit of 1.6 μM for cholesterol with excellent specificity and stability. In addition, the testing results of the as-designed biosensor in patients are highly consistent with clinical diagnostic data, providing a sensitive, reliable, reusable, interference-free, and alternative strategy for clinical cholesterol detection.
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