苯丙素
水杨酸
生物
代谢组
系统获得性抵抗
肉桂酸
植物免疫
植物对草食的防御
茉莉酸
生物化学
植物
拟南芥
基因
生物合成
突变体
代谢物
作者
Willem Desmedt,Wim Jonckheere,Việt Hà Nguyễn,Maarten Ameye,Noemi De Zutter,Karen De Kock,Jane Debode,Thomas van Leeuven,Kris Audenaert,Bartel Vanholme,Tina Kyndt
出处
期刊:Authorea - Authorea
日期:2021-04-30
被引量:1
标识
DOI:10.22541/au.161980656.66482372/v1
摘要
While many phenylpropanoid pathway-derived molecules act as physical and chemical barriers to pests and pathogens, comparatively little is known about their role in regulating plant immunity. To explore this research field, we transiently perturbed the phenylpropanoid pathway through application of the CINNAMIC ACID-4-HYDROXYLASE (C4H) inhibitor piperonylic acid (PA). Using bioassays involving diverse pests and pathogens, we show that transient C4H inhibition triggers systemic, broad-spectrum resistance in higher plant without affecting growth. PA treatment enhances tomato (Solanum lycopersicum) resistance in field and laboratory conditions, thereby illustrating the potential of phenylpropanoid pathway perturbation in crop protection. At the molecular level, transcriptome and metabolome analyses reveal that transient C4H inhibition in tomato reprograms phenylpropanoid and flavonoid metabolism, systemically induces immune signaling and pathogenesis-related genes, and locally affects reactive oxygen species metabolism. Furthermore, C4H inhibition primes cell wall modification and phenolic compound accumulation in response to root-knot nematode infection. Although PA treatment induces local accumulation of the phytohormone salicylic acid, the PA resistance phenotype is preserved in tomato plants expressing the salicylic acid-degrading NahG construct. Together, our results demonstrate that transient phenylpropanoid pathway perturbation is a conserved inducer of plant resistance and thus highlight the crucial regulatory role of this pathway in plant immunity.
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