单元格排序
蛋白质工程
酵母
定向进化
突变
鉴定(生物学)
分类
定向分子进化
计算生物学
流式细胞术
化学
生物化学
细胞
生物
分子生物学
计算机科学
突变体
基因
算法
植物
酶
作者
Byong H. Kang,Brianna M. Lax,K. Dane Wittrup
出处
期刊:Methods in molecular biology
日期:2022-01-01
卷期号:: 29-62
被引量:3
标识
DOI:10.1007/978-1-0716-2285-8_2
摘要
Yeast surface display is a powerful directed evolution method for developing and engineering protein molecules to attain desired properties. Here, updated protocols are presented for purposes of identification of lead binders and their affinity maturation. Large libraries are screened by magnetic bead selections followed by flow cytometric selections. Upon identification and characterization of single clones, their affinities are improved by an iterative process of mutagenesis and fluorescence-activated cell sorting.
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