反应杯
量子点
化学
分散性
生物分子
亮度
表征(材料科学)
荧光
粒子(生态学)
纳米技术
光电子学
分析化学(期刊)
光学
材料科学
色谱法
物理
海洋学
生物化学
地质学
有机化学
作者
Shuo Wang,Lihong Li,Shenghao Jin,Weifeng Liu,Wei Hang,Xiaomei Yan
标识
DOI:10.1021/acs.analchem.7b01885
摘要
Semiconducting quantum dots (QDs) are finding a wide range of biomedical applications due to their intense fluorescence brightness and long-term photostability. Here, we report precise quantification of the fluorescence intensity of single QDs on a laboratory-built high-sensitivity flow cytometer (HSFCM). The nearly uniform illumination of the particles at the intense portions of the radiation field resulted in narrowly distributed signals with high signal-to-noise ratios. By analysis of thousands of QDs individually in as little time as 1 min, intrinsic polydispersity was quickly revealed in a statistically robust manner. Applications of this technique in QD quality assessment, study of metal ion influence, and evaluation of aggregation upon biomolecule coupling are presented. Moreover, an accurate measurement of the QD particle concentration was achieved via single-particle enumeration. HSFCM is believed to provide a powerful characterization tool for QD synthesis and application development.
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