EMS and UV Mutagenesis in Yeast

Abstract Many fundamental biological processes have been elucidated by the isolation and analysis of mutants that are defective in such processes. Therefore, the methods to generate mutants are of great importance in model organisms. This unit describes two protocols for mutagenesis of yeast—using ethyl methanesulfate (EMS) and ultraviolet (UV) light. Each of these methods has been used successfully for many years. Curr. Protoc. Mol. Biol . 82:13.3B.1‐13.3B.5. © 2008 by John Wiley & Sons, Inc.