促炎细胞因子
过氧化物酶体
下调和上调
TLR4型
细胞生物学
脂多糖
肿瘤坏死因子α
化学
生物
炎症
生物化学
免疫学
信号转导
受体
基因
作者
Vijith Vijayan,Srinu Tumpara,Srikanth Karnati,Vannuruswamy Garikapati,Monika Linke,L.A. Kamalyan,Srihari Reddy Mali,Kritika Sudan,Andreas Kollas,Tobias Schmid,Sabine Schulz,Bernhard Spengler,Thomas Weichhart,Stephan Immenschuh,Eveline Baumgart‐Vogt
出处
期刊:Journal of Immunology
[American Association of Immunologists]
日期:2017-02-09
卷期号:198 (6): 2414-2425
被引量:75
标识
DOI:10.4049/jimmunol.1601596
摘要
Abstract Peroxisomes are proposed to play an important role in the regulation of systemic inflammation; however, the functional role of these organelles in inflammatory responses of myeloid immune cells is largely unknown. In this article, we demonstrate that the nonclassical peroxisome proliferator 4-phenyl butyric acid is an efficient inducer of peroxisomes in various models of murine macrophages, such as primary alveolar and peritoneal macrophages and the macrophage cell line RAW264.7, but not in primary bone marrow–derived macrophages. Further, proliferation of peroxisomes blocked the TLR4 ligand LPS-induced proinflammatory response, as detected by the reduced induction of the proinflammatory protein cyclooxygenase (COX)-2 and the proinflammatory cytokines TNF-α, IL-6, and IL-12. In contrast, disturbing peroxisome function by knockdown of peroxisomal gene Pex14 or Mfp2 markedly increased the LPS-dependent upregulation of the proinflammatory proteins COX-2 and TNF-α. Specifically, induction of peroxisomes did not affect the upregulation of COX-2 at the mRNA level, but it reduced the half-life of COX-2 protein, which was restored by COX-2 enzyme inhibitors but not by proteasomal and lysosomal inhibitors. Liquid chromatography–tandem mass spectrometry analysis revealed that various anti-inflammatory lipid mediators (e.g., docosahexaenoic acid) were increased in the conditioned medium from peroxisome-induced macrophages, which blocked LPS-induced COX-2 upregulation in naive RAW264.7 cells and human primary peripheral blood–derived macrophages. Importantly, LPS itself induced peroxisomes that correlated with the regulation of COX-2 during the late phase of LPS activation in macrophages. In conclusion, our findings identify a previously unidentified role for peroxisomes in macrophage inflammatory responses and suggest that peroxisomes are involved in the physiological cessation of macrophage activation.
科研通智能强力驱动
Strongly Powered by AbleSci AI