成骨细胞
破骨细胞
碱性磷酸酶
酸性磷酸酶
斑马鱼
抗酒石酸酸性磷酸酶
细胞凋亡
内科学
流式细胞术
化学
细胞生物学
内分泌学
生物
生物化学
分子生物学
医学
酶
体外
基因
作者
Juan D. Carvajal-Agudelo,A McNeil,Tamara A. Franz‐Odendaal
标识
DOI:10.1016/j.lssr.2023.05.002
摘要
Zebrafish cultured scales have been used effectively to study cellular and molecular responses of bone cells. In order to expose zebrafish scales to simulated microgravity (SMG) and/or vibration, we first determined via apoptosis staining whether cells of the scale survive in culture for two days and hence, we restricted our analyses to two-day durations. Next, we measured the effects of SMG and vibration on cell death, osteoclast tartrate-resistant acid phosphatase, and osteoblast alkaline phosphatase activity and on the number of Runx2a positive cells. We found that during the SMG treatment, osteoclast tartrate-resistant acid phosphatase activity increased on average, while the number of Runx2a positive cells decreased significantly. In contrast, SMG exposure caused a decrease in osteoblast activity. The vibration treatment showed an increase, on average, in the osteoblast alkaline phosphatase activity. This study demonstrates the effect of SMG and vibration on zebrafish scales and the effects of SMG on bone cells. We also show that zebrafish scales can be used to examine the effects of SMG on bone maintenance.
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