DNA‐Programmed Reaction to Evaluate Specific IgE for Allergy Point‐of‐Care Testing

Abstract A DNA‐programmed reaction to evaluate non‐nucleic acids inputs with computation speed (≈30 min) and sensitivity (sub‐picomolar) suitable for analyzing physiologically relevant biomarkers in a one‐pot format and point‐of‐care testing setting is reported. Specifically, a DNA programme based on the proximity‐activation exponential amplification reaction (PEAR) is designed to evaluate specific IgE (sIgE) against Der p 2 implicated in dust mite allergy which affects millions worldwide. In this work, we tailored the molecular components of the input‐to‐oligo barcode conversion module as an AND gate to detect inputs with binding specificity to Der p 2 antigen and is of an IgE isotype. In addition, an in situ biotinylation method is developed to generate amplified oligo barcodes amendable for direct visualization on a lateral flow format. As a proof‐of‐concept demonstration of its potential clinical utility, 21 clinical samples are evaluated by the as‐developed sIgE PEAR programme using the dual readout modality of real‐time fluorescence measurement for precise input quantification and simple lateral flow yes/no answer.