Silica‐Assisted Solid‐Phase Peptide Synthesis (SiPPS)

Non-swelling silica-based resin was used for peptide synthesis. The strategy used is similar to that of solid-phase peptide synthesis (SPPS), referred to as silica-assisted solid-phase peptide synthesis (SiPPS). A 2-h coupling seemed to favor the coupling compared to that of 1-h standard coupling. The use of this non-swelling resin allows a 50% reduction in the consumption of solvents. The strategy was well demonstrated for the synthesis of H-YSSFL-NH₂, linear oxytocin, angiotensin II, and afamelanotide using a silica-based support (Fmoc-Rink amide SiliaBond manufactured from SiliCycle Inc.). The peptides were found to have more than acceptable purity, although there was a loss in overall yields.