自愈水凝胶
明胶
再生医学
干细胞
移植
组织工程
材料科学
脚手架
间充质干细胞
细胞包封
化学
生物医学工程
纳米技术
细胞
高分子化学
细胞生物学
外科
生物化学
医学
生物
作者
Akihiro Nishiguchi,Shima Ito,Kazuhiro Nagasaka,Hiyori Komatsu,Koichiro Uto,Tetsushi Taguchi
标识
DOI:10.1101/2023.07.22.550127
摘要
Abstract Injectable hydrogels are promising carriers for cell delivery in regenerative medicine. However, injectable hydrogels composed of crosslinked polymer networks are often non porous and prevent biological communication with host tissues through signals, nutrients, oxygen, and cells, thereby limiting graft survival and tissue integration. Here we report injectable hydrogels with liquid-liquid phase separation-induced microcapillary networks (µCN) as stem cell-delivering scaffolds. The molecular modification of gelatin with hydrogen bonding moieties induced liquid-liquid phase separation when mixed with unmodified gelatin to form µCN structures in the hydrogels. Through spatiotemporally controlled covalent crosslinking and dissolution processes, porous µCN structures were formed in the hydrogels, which can enhance mass transport and cellular activity. The encapsulation of cells with injectable µCN hydrogels improved cellular adhesion, spreading, migration, and proliferation. Transplantation of mesenchymal stem cells with injectable µCN hydrogels enhanced graft survival and recovered hindlimb ischemia by enhancing material-tissue communication with biological signals and cells through µCN. This facile approach may serve as an advanced scaffold for improving stem cell transplantation therapies in regenerative medicine.
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