间充质干细胞
再生(生物学)
体内
肌腱
组织工程
再生医学
细胞生物学
生物医学工程
材料科学
脚手架
干细胞
医学
解剖
生物
生物技术
作者
Yuzhi Sun,Renwang Sheng,Zhicheng Cao,Chuanquan Liu,Jiaxiang Li,Po Zhang,Yan Li Du,Qingyun Mo,Qingqiang Yao,Qingqiang Yao,Jialin Chen,Jialin Chen,Jialin Chen,Wei Zhang,Wei Zhang,Wei Zhang
出处
期刊:Science Advances
[American Association for the Advancement of Science (AAAS)]
日期:2024-04-26
卷期号:10 (17)
标识
DOI:10.1126/sciadv.adm7164
摘要
Myotendinous junction (MTJ) injuries are prevalent in clinical practice, yet the treatment approaches are limited to surgical suturing and conservative therapy, exhibiting a high recurrence rate. Current research on MTJ tissue engineering is scarce and lacks in vivo evaluation of repair efficacy. Here, we developed a three-dimensional–printed bioactive fiber-reinforced hydrogel containing mesenchymal stem cells (MSCs) and Klotho for structural and functional MTJ regeneration. In a rat MTJ defect model, the bioactive fiber-reinforced hydrogel promoted the structural restoration of muscle, tendon, and muscle-tendon interface and enhanced the functional recovery of injured MTJ. In vivo proteomics and in vitro cell cultures elucidated the regenerative mechanisms of the bioactive fiber-reinforced hydrogel by modulating oxidative stress and inflammation, thus engineering an optimized microenvironment to support the survival and differentiation of transplanted MSCs and maintain the functional phenotype of resident cells within MTJ tissues, including tendon/muscle cells and macrophages. This strategy provides a promising treatment for MTJ injuries.
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