生物复合材料
钴
酶
水溶液中的金属离子
化学
转氨酶
核化学
材料科学
化学工程
金属
工程类
有机化学
复合数
复合材料
作者
Shuzhen Wang,Jiaye Wang,Y. Yuan,Jia-Yan Liu,Yizhou Gao,Qiaoli Hua,Qingcai Jiao,Junzhong Liu,Yang Jin,Hongjuan Zhang,Lina Chen
标识
DOI:10.1016/j.jclepro.2023.136710
摘要
The strategies based on enzyme immobilization into/on metal-organic frameworks (MOFs) have been widely developed, but they still face some challenges in practical applications, such as limited recycle times, low loading efficiency. In this work, aspartate aminotransferase (AspAT), branched-chain aminotransferase (BCAT), polyvinyl pyrrolidone/aspartate (PA), cobalt ions and sodium 2-amino terephthalate were one-pot mixed in water at room temperature, and self-assembled to encapsulate the enzymes into cobalt-amino terephthalate framework (CAF) in 15 min. Compared with free enzymes, the biocomposite (A&B&PA@CAF) exhibited superior performances such as high encapsulation efficiency (55%), reusability (≥12), and resistance capacity to harsh conditions. Meanwhile, the activity recovery (AR) value (59.4%) of the biocomposite without adding polyvinyl pyrrolidone and aspartate was almost half of that (108.2%) of A&B&PA@CAF, and this enhancement was further demonstrated to be from Asp action via 1H-NMR and UV–Vis. To investigate the general applicability of this approach, the other aminases were also de novo immobilized with all their AR values reached more than 117%, even 180% for ω-transaminase. This study provided a sustainable and green approach for clean production of the significant chiral amine compounds.
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