One-step hydrothermal synthesis of near-infrared emission carbon quantum dots as fluorescence aptamer sensor for cortisol sensing and imaging

Fluorescence carbon quantum dots (CQDs) have been widely applied to sensing and bioimaging. In this paper, near-infrared carbon quantum dots (NIR-CQDs) were prepared through a simple one-step hydrothermal approach using reduced glutathione and formamide as raw materials. Based on NIR-CQDs, aptamer (Apt) and graphene oxide (GO) has been applied to fluorescence sensing cortisol. NIR-CQDs-Apt adsorbed to the surface of GO through π-π stacking and an inner filter effect (IFE) occurred between NIR-CQDs-Apt and GO leading to NIR-CQDs-Apt fluorescence "off". The IFE process is disrupted in the presence of cortisol, allowing NIR-CQDs-Apt fluorescence "on". This led us to construct a detection method with excellent selectivity over other cortisol sensors. The sensor can detect cortisol from 0.4 to 500 nM and has a detection limit as low as 0.13 nM. Importantly, this sensor can be used to detect intracellular cortisol with excellent biocompatibility and cellular imaging capabilities, which is promising for biosensing.