Isolation, Purification, and Characterization of Lentinus edodes Polysaccharides Extracted With Subcritical Water Enhanced With Deep Eutectic Solvent

chelating capacity. Moreover, they could reduce the level of reactive oxygen species (ROS) and regulate the activities of malondialdehyde (MDA), catalase (CAT), and superoxide dismutase (SOD) in HepG2 cells, demonstrating strong antioxidant activity. Furthermore, both LEP1 and LEP2 could improve the phagocytic capacity, nitric oxide (NO) release, and the content of interleukin (IL)-6, IL-1β, and tumor necrosis factor-α (TNF-α) in RAW264.7 cells, exhibiting significant immunostimulatory activity. It was worth noting that LEP2 exhibited stronger biological activities than LEP1. Therefore, the SWE enhanced with DES is an ideal method for extracting polysaccharides, which can be further applied to extract other polysaccharides.