化学
抗菌活性
丁香酚
色谱法
精油
气相色谱法
气相色谱-质谱法
薄层色谱法
传统医学
细菌
质谱法
生物
有机化学
医学
遗传学
作者
Weiyi Wen,Haiping Xiang,Huiyun Qiu,Jianwei Chen,Xuemin Ye,Lijia Wu,Zhiqiang Chen,Shengqiang Tong
标识
DOI:10.1016/j.jchromb.2024.124026
摘要
One of the primary components that contribute to Artemisia argyi 's effectiveness is essential oil, which has an exceptional antibacterial effect that has been well documented. The actual cause of its antibacterial activity and associated mechanism, however, are still not completely understood. For the first time, comprehensive two-dimensional gas chromatography coupled with time-of-flight mass spectrometry (2D GC × GC-TOFMS) and thin-layer chromatography-direct bioautography (TLC-DB) were employed to investigate its antibacterial components. The antibacterial properties of A. argyi essential oil were investigated, and the antibacterial activity of six compounds was evaluated, using Staphylococcus aureus (S. aureus) and Escherichia coli (E. coil) as test microorganisms. TLC-direct bioautography was used to screen two bioactive clusters. Following 2D GC × GC-TOFMS identification of bioactive clusters, six compounds were evaluated for in vitro antibacterial activity verification. All the components tested displayed antibacterial action. Results showed that α-terpineol and eugenol had high potent antibacterial activity (MIC<0.62 mg/mL, IC50<2.00 mg/mL). For complex essential oils from traditional Chinese medicine, this method is efficient for quick screening and identifying antibacterial compounds.
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