Scanning electron microscopic observations of apical root surfaces of teeth with apical periodontitis

Abstract The aim of this study was to observe apical fool surfaces of teeth with chronic periapical lesions. Five premolars and three molars with radiographically demonstrable periapical lesions were extracted and fixed in 2.5% phosphate‐buffered glutaraldehyde solution For 9 days. The apical 3‐mm portion of 17 roots was removed and prepared for scanning electron microscope. Lacunar resorption zones were frequently observed on the root surfaces. Bacteria and yeast cells were detected in some of the lacunae. Periapical bacterial plaque with a smooth structure was present mostly around the main apical foramen. Cementum‐like tissue deposits indicative of repair were seen adjacent to some resorption areas. Clastic cells lightly attached to crater‐like depressions with linger‐like projections were observed laterally on the specimens. Current research should be focused on new procedures to eliminate extraradicular microrganisms and periapical bacterial plaque in persistent periapical infections.