生物
遗传增强
基因
重组DNA
病毒学
体内
腺病毒基因组
腺病毒科
病毒复制
复制(统计)
遗传学
病毒
作者
Hanns Lochmüller,Ágnes Jáni,Johnny Huard,Stephen M. Prescott,Maude Simoneau,Bernard Massie,George Karpati,Gyula Acsádi
出处
期刊:Human Gene Therapy
[Mary Ann Liebert]
日期:1994-12-01
卷期号:5 (12): 1485-1491
被引量:189
标识
DOI:10.1089/hum.1994.5.12-1485
摘要
Early region 1 (E1)-deleted human adenovirus (AV) recombinants have been shown to be powerful tools of gene transfer in vivo and in vitro and are considered for application in human gene therapy. We could detect increasing titers of E1-containing adenovirus in two independent E1 + E3-deleted recombinant AV stocks during multiple passages in 293 cells, most likely due to a recombinant event with the host cell genome. We show the deleterious effects of this E1-containing, mostly replication-competent AV subpopulation in vivo and compare different screening methods of AV stocks for its detection. These considerations are important for the safety of human gene therapy trials.
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