上皮-间质转换
TLR4型
癌症研究
MAPK/ERK通路
转移
信号转导
脂多糖
Toll样受体
间充质干细胞
体外
化学
细胞生物学
受体
生物
免疫学
医学
内科学
癌症
先天免疫系统
生物化学
作者
Hangyu Li,Yan Li,Dan Liú,Jingang Liu
出处
期刊:Tumor Biology
[SAGE]
日期:2014-07-23
卷期号:35 (10): 10429-10435
被引量:48
标识
DOI:10.1007/s13277-014-2347-5
摘要
The endotoxin level in the portal and peripheral veins of hepatocellular carcinoma (HCC) patients is higher and lipopolysaccharide (LPS), has been reported to inhibit tumor growth. However, in this study, we found that LPS-induced Toll-like receptor 4 (TLR4) signaling was involved in tumor invasion and the molecular mechanism was investigated. The HCC cells were used to study the invasion ability of LPS-induced HCC cells and the epithelial–mesenchymal transition (EMT) in vitro. The in vitro experiments demonstrated that LPS could significantly enhance the invasive potential and induce EMT in HCC cells with TLR4 dependent. Further studies showed that LPS could directly activate JNK/MAPK signaling through TLR4 in HCC cells. Interestingly, blocking JNK/MAPK signaling significantly inhibited EMT occurrence. Our results indicate that TLR4/JNK/MAPK signaling is required for LPS-induced EMT, tumor cell invasion and metastasis, which provide molecular insights for LPS-related pathogenesis and a basis for developing new strategies against metastasis in HCC.
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