医学
糖尿病肾病
肾
CD8型
肾病
CD20
流式细胞术
内科学
内分泌学
糖尿病
免疫组织化学
病理
免疫系统
免疫学
作者
Jae‐Young Moon,Kyung-Hwan Jeong,Tae-Won Lee,Chun-Gyoo Ihm,Sung Jig Lim,Sang-Ho Lee
出处
期刊:American Journal of Nephrology
[S. Karger AG]
日期:2012-01-01
卷期号:35 (2): 164-174
被引量:113
摘要
Recent evidence has shown that an inflammatory process is involved in the development and progression of diabetic nephropathy. This study examined the impact of activated intrarenal lymphocytes in this inflammatory process.We studied T cell recruitment in mice with streptozotocin (STZ)-induced diabetes by flow cytometry and immunohistochemistry. The kidney biopsy specimens from patients with type 2 diabetes mellitus and diabetic nephropathy were evaluated by immunohistochemistry.In flow cytometry, intrarenal CD3+ T cells were significantly increased in proteinuric mice at 20 weeks after STZ injection. However, the population of T cells and B cells in diabetic spleen was not different from that of control mice. Immunohistochemistry also showed a marked infiltration of interstitial CD4+, CD8+ T cells in diabetic kidney. Interferon-γ (IFN-γ) and tumor necrosis factor-α (TNF-α) mRNA expression was significantly increased in diabetic mouse kidney compared with controls. Interestingly, flow cytometry analysis of kidney-derived mononuclear cells from diabetic mice showed significantly increased production of IFN-γ and TNF-α by CD3+ T cells. Type 2 diabetic patients also showed a marked increase in CD4+, CD8+ and CD20+ cells in interstitium, and the number of CD4+ and CD20+ cells correlated with the amount of proteinuria.Our results clearly showed that aberrant intrarenal infiltration and the activation of T cells in interstitium are the underlying immunopathological mechanisms of diabetic kidney injury.
科研通智能强力驱动
Strongly Powered by AbleSci AI