鼻咽癌
转移
异位表达
癌症研究
生物
细胞培养
体内
免疫组织化学
小RNA
污渍
病理
医学
癌症
内科学
免疫学
基因
放射治疗
遗传学
生物技术
生物化学
作者
Na Liu,Ling TANG,Ying Sun,Rui Cui,Hui Yun Wang,Bi Huang,Qiuming He,Wei Jiang,Jun Ma
出处
期刊:Cancer Letters
[Elsevier]
日期:2013-02-01
卷期号:329 (2): 181-188
被引量:102
标识
DOI:10.1016/j.canlet.2012.10.032
摘要
Based on microarray analysis, we previously reported that miR-29c is significantly downregulated in nasopharyngeal carcinoma (NPC). However, little is known about the effect and molecular mechanisms of action of miR-29c deregulation during the development and progression of NPC. Quantitative RT-PCR demonstrated that miR-29c was significantly downregulated in NPC cell lines and clinical specimens. Wound healing, Transwell migration and lung metastasis assays demonstrated that ectopic expression of miR-29c inhibited NPC cell migration and invasion in vitro and suppressed the formation of lung metastases in vivo. T cell lymphoma invasion and metastasis 1 (TIAM1) was confirmed as a miR-29c target gene using luciferase reporter assays, quantitative RT-PCR and Western blotting. Ectopic expression of TIAM1 significantly promoted the migration and invasion of SUNE-1 cell line stably overexpressing miR-29c. The prognostic value of TIAM1 was analyzed in 217 NPC patients using immunohistochemistry. Strikingly, patients with high TIAM1 expression had poorer overall, disease-free and distant metastasis-free survival than patients with low TIAM1 expression. Furthermore, multivariate Cox regression analysis revealed that TIAM1 could serve as an independent prognostic factor in NPC. The newly identified miR-29c/TIAM1 pathway further elucidates the molecular mechanisms regulating invasion and metastasis in NPC, and may provide novel prognostic and treatment strategies for NPC patients.
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