Determination of Ginsenoside Rg_1 Rb_1 and Sanchinoside R_1 in the Panax Notoginseng Saponions Extracts by HPLC

Aim A high-performance liquid chromatography(HPLC)method was developed for determination of sanchinoside R1,ginsenoside Rg1,ginsenoside Rb1 in panax notoginseng saponions extracts.Methods RP-HPLC method was employed on ODS column.Sanchinoside R1,ginsenoside Rg1,ginsenoside Rb1 were eluated with acetonitrile(A)-H2O(B)(A∶B,V/V,23∶77 from 0 to 5min,23~25∶77~75 from 5 to 10min,25~40∶75~60 from 10 to 35min,40~23∶60~77 from 35 to 36min,23∶77 from 36 to 40min).Results Sanchinoside R1,ginsenoside Rg1,ginsenoside Rb1were detected at 203nm.The HPLC method showed a good linear relationship in the range of 25.03～150.2mg·L-1 for sanchinoside R1,101.6~609.6 mg·L-1 for ginsenoside Rg1 and 104.7~628.2mg·L-1 for ginsenoside Rb1.Their coefficients of determination(r)were 0.999 9.Conclusion This HPLC method was simple,accurate and instant,which can be used for content determination of panax notoginseng saponions extracts.