The activation and motility of spermatozoa from Chinook salmon collected by dissection were studied under different reactivators,ASP,pH and temperature.The results were as follows: sperm motility in BSS is higher than that in water(P0.05).Sperm motility of one fish is better than that of mixed milt(P0.05).In ASP A,B and C,ASP C is the best to preserve spermatozoa(P0.05).The pH value to help preserve strong motility of the spermatozoa is pH 8.19~8.40,and spermatozoa motility is the highest under the conditions of pH 8.40. When temperature is 4℃,sperm motility is the highest.The optimum temperature and pH for the highest fertilization of Chinook salmon are also 4 and 8.4 respectively,consistent with the conditions making the vitality of the spermatozoa highest.