The research reveals the enrichment of ANAMMOX bacterium and the effect of inorganic carbon on ANAMMOX in anaerobic sequencing bath reactor(ASBR).The enrichment of ANAMMOX bacterium was carried out in a 8.0 L ASBR,and the effect of inorganic carbon on ANAMMOX was carried out in a 4.0 L ASBR.Research results showed the first signs of ANAMMOX activity after 55 days cultivation;ANAMMOX bacterium became the dominant species at 85 days;At 140 days,the system ran steadily,NH4+-N and NO2——N removal rate were very high,and the color of sludge changed from gray to pink gradually.The ANAMMOX activity increased as the influent bicarbonate concentration increased from 1.0 to 1.4 g/L,and then,was inhibited as bicarbonate concentration increased to 2.0 g/L.However,the activity could be restored by the reduction of bicarbonate concentration to 1.0 g/L.