Brucellosis is still prevalent in the world today. Brucella maris have been found except Brucella abortus, Brucella melitensis, Brucella suis, Brucella ovies and Brucella canies. Not only isolated organ, but also more sensitivity methods such as PCR and DNA probe have been applied to confirm. ELISA has been widely used to detect brucellosis in the world. Two new rough type Brucella vaccine, RB51 and M111, are more effective for preventing brucellosis.