Expression of Bovine Follicle Stimulating Hormone Using Pichia pastoris

Objective: To express recombinant bovine follicle stimulating hormone (bFSH) using Pichia pastoris.Methods: Two DNA sequences coding for subunit α and subunit β with the C-terminal end of 6×his-tag were optimized based on synonymous codons usage preferred by the methylotropic yeast P.pichia.These genes were synthesized based on a polymerase chain reaction(PCR).The vector pHIL-S-bFSH was constructed and transformed Pichia pastoris.The expressed protein was analyzed using ELISA and Western blot.Results: The expression level of recombinant bFSH was 0.26mg/L,the molecular was 35kDa.Conclusion: The recombinant bovine FSH was successfully expressed using P.pastoris.