Construction of Prokaryotic Expression Vector for Therapeutic Alzheimer Disease Vaccine

Objective To construct a prokaryotic expression vector for therapeutic Alzheimer disease(AD) vaccine.MethodsLigating the DNA sequence encoding β-amyloid protein(Aβ) 1-15 to that encoding murine chemotactic factor BCA-1 by PCR to obtain synthetic gene AZM.Insert AZM gene into genetically modified prokaryotic expression vector pRSET/no His and identify the recombinant plasmid pREST-AZM by restriction analysis and DNA sequencing.Results Both restriction analysis and DNA sequencing proved that AZM gene was correctly inserted into expression vector pREST/no His.Conclusion The prokaryotic expression vector of Aβ42 subunit vaccine was successfully constructed.