Proliferation and differentiation of neural stem cells in hippocampus dentate gyrus in adult and aged rats after intracerebral hemorrhage

Objective To investigate the difference of the proliferation and differentiation of neural stem cells(NSCs) in the hippocampus dentate gyrus between adult and aged rats after intracerebral hemorrhage(ICH),to explore the variation of NSCs after ICH.Methods ICH models were established by injecting collagenase Ⅶ into the brain of rats.Proliferating cells were labeled by BrdU abdominal cavity injection.Immunohistochemical single and double staining with antibodies against BrdU,NeuN and GFAP were used to determine proliferation and differentiation of hippocampus dentate gyrus.Results In non-ICH rats,BrdU positive cells in hippocampus dentate gyrus of adult rats were much more than those of aged rats.After ICH,BrdU positive cells in hippocampus dentate gyrus of rats were significantly increased.7 d group rats had reached its peak.BrdU positive cells in hippocampus dentate gyrus of adult rats were much more than those of aged rats all the time.In non-ICH rats,a little of BrdU/NeuN and BrdU/GFAP double positive cells were found in the hippocampus dentate gyrus.After ICH,the quantity of double positive cells were increased obviously.BrdU/GFAP double positive cells of aged rats were much more than those of adult rats,but BrdU/NeuN double positive cells of aged rats were less than those of adult rats.Conclusions After ICH,NSCs in hippocampus dentate gyrus of rats are activated.Proliferation and differentiation of NSCs in hippocampus dentate gyrus of adult rats are stronger than those of aged rats.