Identification of phenolic constituents in Radix Salvia miltiorrhizae by liquid chromatography/electrospray ionization mass spectrometry

Abstract The phenolic components from Radix Salvia miltiorrhizae Bunge, a well‐known herbal medicine (Dan‐Shen in Chinese), have been investigated by high‐performance liquid chromatography/electrospray ionization tandem mass spectrometry (HPLC/ESI‐MS/MS). HPLC analyses were performed on a reversed‐phase C 18 column using gradient elution. In the ESI mass spectra a predominant [M–H] − ion was observed in negative mode and provided molecular mass information. ESI‐MS/MS spectra of the [M–H] − ions were used for structural analysis, based on the spectra of standards. It was found that caffeic acid and its monomeric analogs containing a carboxyl group readily lost CO 2 , while dimers, trimers and tetramers of caffeic acid expelled successively danshensu or caffeic acid or their esters. Twenty‐eight phenolic compounds in S. miltiorrhizae were characterized, of which eight compounds were positively identified by comparison with standards. The remaining twenty phenolics for which standards were not available were tentatively identified based on their UV spectra and MS/MS fragmentation characteristics. Copyright © 2006 John Wiley & Sons, Ltd.