生物分析
寡核苷酸
计算生物学
生化工程
纳米技术
计算机科学
化学
色谱法
生物
材料科学
工程类
DNA
生物化学
作者
Laixin Wang,Chengjie Ji
出处
期刊:Bioanalysis
[Newlands Press Ltd]
日期:2016-01-01
卷期号:8 (2): 143-155
被引量:29
摘要
Technical advances and demands for high-throughput accurate quantification of oligonucleotide therapeutics and biomarkers in pharmaceutical research and clinical diagnosis have aided evolution in quantitative bioanalysis of oligonucleotides. Many bioanalytical methods are available for absolute quantification of oligonucleotides in biological matrices. They can be broadly classified into two categories: hybridization-based assays commonly used by molecular biologists and chromatographic assays routinely used by chemists. Each category has its own advantages and disadvantages for specific applications. This review summarizes the mechanisms and applications of some of the current most commonly used techniques in each category.
科研通智能强力驱动
Strongly Powered by AbleSci AI