A Method for Accelerating the Maturation of Toxocara cati Eggs

Background: The effect of temperature and humidity on the maturation of Toxocara cati eggs in an in vitro system was investigated. Methods: Suspensions of Toxocara cati eggs, with 5% formalin/saline or 2.5% formalin/ringer were prepared and maintained at 37 °C under 40% humidity or at 25 °C under 98% humidity for 3 weeks for egg development. Results: The suspension sample mixed by 2.5% formalin/ringer and maintained at 25 oC and 98% humidity could fully embryonate the eggs of Toxocara cati in 3 weeks. Conclusion: The main advantage of this method is the increase of recovery and also reducing of the eggs maturation time.