生物
贾第虫
蓝氏贾第鞭毛虫
基因分型
聚合酶链反应
核糖体DNA
核糖体RNA
系统发育树
16S核糖体RNA
原生动物
微生物学
遗传学
基因型
基因
作者
Tai Soon Yong,S.-J. Park,Ui Wook Hwang,Hea-Eun Yang,K.-W. Lee,Duk Young Min,Han-Jong Rim,Y. Wang,Zheng Fan
出处
期刊:Journal of Parasitology
[BioOne (American Society of Parasitologists)]
日期:2000-01-01
卷期号:86 (4): 887-887
被引量:44
标识
DOI:10.1645/0022-3395(2000)086[0887:goglif]2.0.co;2
摘要
Genetic characterization of a total of 15 Giardia lamblia isolates, 8 from Anhui Province, China (all from purified cysts) and 7 from Seoul, Korea (2 from axenic cultures and 5 from purified cysts), was performed by polymerase chain reaction amplification and sequencing of a 295-bp region near the 5' end of the small subunit ribosomal DNA (eukaryotic 16S rDNA). Phylogenetic analyses were subsequently conducted using sequence data obtained in this study, as well as sequences published from other Giardia isolates. The maximum parsimony method revealed that G. lamblia isolates from humans in China and Korea are divided into 2 major lineages, assemblages A and B. All 7 Korean isolates were grouped into assemblage A, whereas 4 Chinese isolates were grouped into assemblage A and 4 into assemblage B. Two Giardia microti isolates and 2 dog-derived Giardia isolates also grouped into assemblage B, whereas Giardia ardeae and Giardia muris were unique.
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