Antioxidant activity detection of Astragalus membranaceus and Codonopsis pilosula ethanol extracts by DPPH method.

Using the assay systems of spectrophotometric method,we determined the ability of scavenging effect on DPPH of Astragalus membranaceus and Codonopsis pilosula ethanol extracts from different habitats to investigate the antioxidant activity of A.membranaceus and C.pilosula.The scavenging radical capacities on DPPH of A.membranaceus from Weiyuan Gansu,Longxi Gansu,Shulan Jilin and Hunyuan Shanxi,were 21.38%,13.53%,35.09% and 20.18%,respectively.The scavenging radical capacities on DPPH of C.pilosula from Fengxian Shaanxi,Lingchuan Shanxi,and Nanzheng Shaanxi,were 17.56%,9.87% and 8.03%,respectively.The IC50 of A.membranaceus and C.pilosula were 0.24 and 0.85 mg·mL-1,respectively.It was shown that the antioxidant activity was higher in A.membranaceus than that in C.pilosula.