Angelica dahurica-derived exosome-like nanovesicles as bioactive nanomaterials for high-glucose-impaired tissue repair

Plant-derived exosome-like nanovesicles have source-dependent compositions that may support tissue repair. Angelica dahurica -derived exosome-like nanovesicles (AD-ELNs) were enriched by differential centrifugation and ultracentrifugation and evaluated under high-glucose stress. The preparation showed rounded vesicle-like morphology, a mean hydrodynamic diameter of approximately 102.3 nm, a PDI of 0.182, and a negative surface charge. Nanoparticle tracking analysis of three independently prepared batches gave a mean particle diameter of 141.1 ± 1.4 nm. AD-ELNs showed favorable biocompatibility, enhanced endothelial network formation and fibroblast scratch closure, and partially restored zebrafish tail-fin regeneration in a glucose-induced diabetic wound model. Untargeted metabolomics identified putative furanocoumarins, including isoimperatorin and bergaptol. Direct treatment with either standard improved tail-fin repair under high-glucose conditions. Tail-fin qPCR showed increased egfra and egfrb expression with both compounds, whereas isoimperatorin also increased akt1 , pik3ca , and mtor expression. Network predictions and transcriptomic analyses linked these findings with immune, stress, lipid/membrane, and extracellular matrix responses. The results support a relationship between the plant-associated chemical background and repair activity while identifying candidate transcriptional responses for further mechanistic testing.