Blocking Antibody Induced by Immunization with Hypoallergenic Derivatives of Scy p 1 Alleviated Mud Crab Allergy Symptoms

Crab triggers severe IgE-mediated allergic reactions. A rabbit anti-IgG antibody induced by hypoallergenic derivatives of Scy p 1 (mtScy p 1) effectively blocked wild-type Scy p 1 (wtScy p 1) binding to serum IgE; however, its protection against allergic symptoms needs to be further studied. In this paper, rabbit anti-mtScy p 1 IgG antibody could inhibit wtScy p 1-induced basophil degranulation, mainly due to the overlap of 2 IgG conformational epitopes and 4 IgG linear epitopes with IgE epitopes. In Balb/c mice, compared with the wtScy p 1 group, the levels of IgE and IgG1, B cells, IL-4, and IL-17A were significantly decreased, whereas the production of IgG2a, TCR-β cells, Foxp3 cells, IFN-γ, and IL-10 was obviously increased in the rabbit anti-mtScy p 1 IgG antibody group, thereby alleviating the allergic response by modulating Th1/Th2 and Th17/Treg balance. These findings indicated that rabbit anti-mtScy p 1 IgG antibody holds promise for an immunotherapeutic regimen.