人脑
切片制备
神经科学
哺乳动物大脑
脑组织
生物
人类疾病
电生理学
医学
病理
疾病
作者
Aniella Bak,Henner Koch,Karen M.J. van Loo,Katharina Schmied,Birgit Gittel,Yvonne Weber,Jonas Ort,Niklas Schwarz,Simone C. Tauber,Thomas V. Wuttke,Daniel Delev
标识
DOI:10.1016/j.jneumeth.2023.110055
摘要
The investigation of the human brain at cellular and microcircuit level remains challenging due to the fragile viability of neuronal tissue, inter- and intra-variability of the samples and limited availability of human brain material. Especially brain slices have proven to be an excellent source to investigate brain physiology and disease at cellular and small network level, overcoming the temporal limits of acute slices. Here we provide a revised, detailed protocol of the production and in-depth knowledge on long-term culturing of such human organotypic brain slice cultures for research purposes. We highlight the critical pitfalls of the culturing process of the human brain tissue and present exemplary results on viral expression, single-cell Patch-Clamp recordings, as well as multi-electrode array recordings as readouts for culture viability, enabling the use of organotypic brain slice cultures of these valuable tissue samples for basic neuroscience and disease modeling (Fig. 1).
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