生物
胚泡
胚胎
劈理(地质)
男科
细胞生物学
胚胎发生
断裂(地质)
医学
古生物学
作者
Heqiang Li,Jing Gao,Jingyi Wang,Baobao Zhao,Xiaonan Ma,Bihan Zhang,Cuicui Lv,Han Qiao,Yongsheng Wang,Qing Su-zhu
标识
DOI:10.1093/biolre/ioae024
摘要
Abstract Accumulating evidence indicates that paternally-derived miRNAs play a crucial role in the development of early embryos and are regarded as the key factor in the successful development of somatic cell cloned embryos. In our previous study, bta-miR-301a was found to be highly expressed in bovine sperm, and was delivered into oocytes during fertilization. In this study, bioinformatics, dual luciferase reporter assays, rescue experiments and gain- and loss-of-function experiments indicated that ACVR1 is the target gene of bta-miR-301a in early bovine embryos. By microinjecting bta-miR-301a mimic into embryos of parthenogenetic or somatic cell nuclear transfer, we observed that bta-miR-301a prolonged the first cleavage time of the embryos and increased the blastocyst formation rate. Thus, this study provides preliminary evidence that bta-miR-301a influences remodeling of the microfilament skeleton, prolongs the first cleavage time, and improves the developmental competence of embryos by negatively regulating ACVR1 translation.
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